Reagent	Final concentration	Amount
High glucose DMEM (with L-Glutamine and Sodium Pyruvate)	n/a	40 mL
Fetal Bovine Serum (FBS)	10% (v/v)	5 mL
Ham’s F-12 Nutrient Mix	10% (v/v)	5 mL
Total	n/a	50 mL