| Reagent | Final concentration | Amount |
|---|---|---|
| Neurobasal-A | n/a | 48.5 mL |
| B-27 (50×) | 2% (v/v) | 1 mL |
| GlutaMAX (100×) | 1% (v/v) | 500 μL |
| Total | n/a | 50 mL |
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