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. 2022 Jul 16;3(3):101542. doi: 10.1016/j.xpro.2022.101542

Figure 1.

Figure 1

Preparation of the adult brain explant

(A) Diagrams showing how the pigmentless adult brain explant is prepared.

(B and C) Detailed procedure for the dissection of the adult zebrafish brain. (C) Optional steps. Red marks indicate optional steps.

(D) Diagrams showing the key steps for the preparation of a brain explant from a pigmented zebrafish. The black lines delineate the border of the adult brain (B3, D2). The dotted straight lines label the cutting sites. Red arrows indicate pulling directions. Black arrows mark brain regions. Anterior is to the right in all panels. Abbreviation: Telencephalon (Tel), olfactory bulb (OB), choroid plexus (ChP), pineal gland (Pi), anterior (A), posterior (P), left (L) and right (R). Scale bar: 100 μm.