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. 2022 Jul 5;13:888522. doi: 10.3389/fphar.2022.888522

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Copyright © 2022 Cao, -Zhu, Zhang, Meng, Wu, Wang, Wu, Zou, Fang, An and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TBMA and hordenine regulated DRD2 DNA methylation. (A,B) PRL, DRD2, and DNMT protein expression by western blotting following treatment with different risperidone concentrations (n = 3). (C) PRL, DRD2, and DNMT mRNA expression levels by RT-PCR following different risperidone concentrations (n = 3). (D,E) WB was used to detect PRL, DRD2, and DNMT protein expression by western blotting following TBMA or hordenine treatment of MMQ cells (n = 3). (F) was used to detect the mRNA levels of PRL, DRD2, and DNMT mRNA expression by RT-PCR following TBMA or hordenine treatment of MMQ cells (n = 3). Data are presented as the mean ± SD (^# p < 0.05 and ^## p < 0.01 versus the control group; *p < 0.05 and **p < 0.01 versus the Ris group). Hor = hordenine; Ris = risperidone; TBMA = total barley maiya alkaloids.