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. 2022 Jul 5;16:917713. doi: 10.3389/fncel.2022.917713

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Copyright © 2022 Sheng, Zhao, Huang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Raw image registration by ORCA and other software. (A) Z-project of raw images and zoom-in view. (B) Registration speed of different methods measured in frames per second. (C) Top panel: z-project of zoomed-in view after registration; bottom: calculated shifts in x and y directions. Scale bar, 2 μm. (D) Z-project of the simulated movie. (E) Quantification of registration accuracy with simulated data in panel (D). (F) Z-project of zoomed-in view after registration of simulated movie. Scale bar, 2 μm.