Table 4.

Molecular genetic findings of uterine corpus mesonephric-like adenocarcinomas.

Case No.	Case 1	Case 2	Case 3	Case 4
Mutations	PTEN: exon 8 c.974T>G(p.L325R). VAF:78.85% Uncertain significance	DUSP2:exon2 c.504C>G(p.Y168*). VAF:77.55% Uncertain significance	PIK3CA:exon9 c.1637A>G(p.Q546R). VAF:52.6% Likely pathogenic	PIK3CA:exon20 c.3145G>C(p.G1049R). VAF:45.9% Likely pathogenic
	KRAS: exon2 c.35G>C(p.G12A). VAF:72.29% Likely pathogenic	KRAS: exon2 c.35G>T(p.G12V). VAF:69.45% Likely pathogenic	SMARCB1:exon8 c.1091_1093del(p.K364del).VAF:14.87% Uncertain significance	CHD4:exon25 c.3740T>C(p.I1247T). VAF:2.03% Uncertain significance
	ATM:exon50 c.7466C>T(p.S2489F). VAF:35.14% Uncertain significance	SMAD4:exon8 c.913C>A(p.H305N).VAF:31.61% Uncertain significance	MYCN:exon2 c.131C>T(p.P44L). VAF:11.52% Uncertain significance
	ATM:exon10 c.1372_1382dup (p.E461Dfs*16). VAF:21.69% Uncertain significance	PIK3CA:exon1 c.277C>T(p.R93W). VAF:29.91% Uncertain significance
	KMT2C:intron7 c.1012+1G>A. VAF:8.67% Uncertain significance	DPYD:exon21 c.2737A>G(p.I913V). VAF:23.62% Likely benign
	TMPRSS2:exon3 c.230A>C(p.H77P). VAF:8.11% Uncertain significance	PTCH1:exon23 c.4282G>A(p.E1428K).VAF:22.68% Uncertain significance
		TACC3:exon9 c.1787G>A(p.R596Q). VAF:20.33% Uncertain significance
CNV			NKX2-1 (CN:4.2)
TMB (per megabase)	7.4 mutations	7.4 mutations	3.2 mutations	2.1 mutations
Microsatellite analysis	No mutations in DNA mismatch repair genes were detected

CN, copy number; CNV, copy number variation; TMB, tumor mutational burden; VAF, variant allele frequency.