FIGURE 4.
The representative photomicrographs of H&E staining (A), Nissl staining (B) and immunofluorescence staining of GFAP (green) (C,D) in the DG region of the hippocampus (n = 4 in each group). In photomicrographs of immunofluorescence, astrocytes were positive for GFAP with ramified branches. The images of the scale bar represent 100 μm, magnification = 200X. Values are presented as means ± SEM. *p < 0.05 when compared with the control group; #p < 0.05 when compared with the D-gal group. DG, dentate gyrus; GFAP, glial fibrillary acidic protein; SEM, standard error of mean.