Table 1.

Mutations and amino acid substitutions at the six positions.

Protein (region)	Position (nt)	Amino acid (aa)	Wildtype nucleotide	Variant nucleotide	Change in aa
E (Domain I)	1,350	T188	C	A	No
NS3 (Helicase)	5,990	E490	A	C	E → A
NS5 (MTase)	7,812	G81	U	G	No
NS5 (RdRp)	8,919	C450	U	G	C → W
NS5 (RdRp)	9,420	A617	C	A	No
NS5 (RdRp)	9,938	T790	C	A	T → K

MTase, Methyltransferase; RdRp, RNA-dependent RNA polymerase; No, no mutation (synonymous/silent mutation); nt, nucleotide; aa, amino acid. Location of positions from LoFreq with the viral genome’s nucleotide position number, viral protein amino acid number and amino acid, wildtype and variant nucleotide at the position, and any amino acid change which occurred when substituting wildtype nucleotide with the variant nucleotide. Variations were found to have a higher frequency in fatal cases than in mild cases.