TABLE 3.
Core lipid composition of the biomass (totCLs) and of the total lipid extract (CLs from IPLs) of T. barophilus.
| Diethersa |
Tetraethersa |
Total |
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| DGD I | PSGD XII | GDGT0 XVIII | GTGT0 XXII | |||||||||||
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| μ g | fg cell–1 | Rel% | μ g | fg cell–1 | Rel% | μ g | fg cell–1 | Rel% | μ g | fg cell–1 | Rel% | fg cell–1 | D/T | |
| TotCLs | 22.2 | 0.1 | 28 | 6.1 × 10–2 | 3.1 × 10–4 | Traces | 56.8 | 0.3 | 72 | 0.4 | 2.2 × 10–3 | Traces | 0.4 | 0.39 |
| CLs from IPLs | 14.6 | 7.3 × 10–2 | 80 | 4.2 × 10–2 | 2.1 × 10–3 | Traces | 3.6 | 1.8 × 10–2 | 20 | 3.1 × 10–2 | 1.5 × 10–4 | Traces | 9.1 × 10–2 | 4.0 |
DGD, dialkyl glycerol diethers; PSGD, phytanylsesterterpanyl glycerol diethers; GDGT0, glycerol dialkyl glycerol tetraethers with no cyclopentane ring; GTGT0, glycerol trialkyl glycerol tetraethers with no cyclopentane ring; D/T, diethers over tetraethers ratio; Rel%, molar relative proportion; μg, absolute lipid quantity in μg; fg cell–1, cellular lipid abundance in fg cell–1 calculated with an average cell number of 2.0 × 108 cell mL–1; ND, not detected. Traces, < 1%. aRelative proportions account for protonated adducts in APCI positive mode and were calculated assuming a response factor of 0.42 for diethers and 0.57 for tetraethers relative to the internal standard C46-GTGT, or of 0.74 for diethers relative to tetraethers (refer to methods).