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. 2022 Jul 5;13:869479. doi: 10.3389/fmicb.2022.869479

TABLE 3.

Core lipid composition of the biomass (totCLs) and of the total lipid extract (CLs from IPLs) of T. barophilus.

Diethersa
Tetraethersa
Total
DGD I PSGD XII GDGT0 XVIII GTGT0 XXII





μ g fg cell–1 Rel% μ g fg cell–1 Rel% μ g fg cell–1 Rel% μ g fg cell–1 Rel% fg cell–1 D/T
TotCLs 22.2 0.1 28 6.1 × 10–2 3.1 × 10–4 Traces 56.8 0.3 72 0.4 2.2 × 10–3 Traces 0.4 0.39
CLs from IPLs 14.6 7.3 × 10–2 80 4.2 × 10–2 2.1 × 10–3 Traces 3.6 1.8 × 10–2 20 3.1 × 10–2 1.5 × 10–4 Traces 9.1 × 10–2 4.0

DGD, dialkyl glycerol diethers; PSGD, phytanylsesterterpanyl glycerol diethers; GDGT0, glycerol dialkyl glycerol tetraethers with no cyclopentane ring; GTGT0, glycerol trialkyl glycerol tetraethers with no cyclopentane ring; D/T, diethers over tetraethers ratio; Rel%, molar relative proportion; μg, absolute lipid quantity in μg; fg cell–1, cellular lipid abundance in fg cell–1 calculated with an average cell number of 2.0 × 108 cell mL–1; ND, not detected. Traces, < 1%. aRelative proportions account for protonated adducts in APCI positive mode and were calculated assuming a response factor of 0.42 for diethers and 0.57 for tetraethers relative to the internal standard C46-GTGT, or of 0.74 for diethers relative to tetraethers (refer to methods).