Table 3.
The sequence of tested primers applied in real-time PCR analysis.
| Gene | Forward 5′ > 3′ | Reverse 5′ > 3′ | Acc. No. | Ampl. size (bp) |
|---|---|---|---|---|
| IL-1β | CCAGAAAGTGAGGCTCAACA | GTAGCCCTTGATGCCCAGT | KJ891452 | 591 |
| IL10 | CTGCACTTCTCTGAGCTGCT | CTTCCTCCTCCTCATCAGCA | NM_001004414 | 528 |
| GHR | CAGCTGCTGTTGACCTTGG | CCAGTGCCAAGGTCAACAG | AH002706 | 4829 |
| IGF-1 | TACCACCAACTCAGAGCAGG | GGTTTTCTTCTGCCTTGGGG | NM_001085376 | 8975 |
| GAPDH | GACGTGCAGCAGGAACACTA | TCTCCATGGTGGTGAAGACA | AJ312193 | 602 |
| YWHAZ | TTGCTGCTGGAGATGACAAG | CTTCTTGATACGCCTGTTG | GCA_016699485.1 | 104 |
IL-1β, interleukin 1 Beta; IL10, interleukin 10; GHR, growth hormone receptors: IGF-1, insulin-like growth factor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide.