Plat-E medium

Reagent	Final concentration	Amount
Fetal Bovine Serum	10% (v/v)	5 mL
L-Glutamine (200 mM)	2 mM	0.5 mL
DMEM	n/a	44.5 mL
Total	n/a	50 mL

Prepare in a biological safety cabinet. Store at 4°C for up to one month.