REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
DMEM	Thermo Fisher Scientific	Cat# 11960044
Iscove’s Modified Dulbecco’s Medium (IMDM)	Sigma-Aldrich	Cat# I3390
Opti-MEM™ I Reduced Serum Medium	Life Technologies	Cat# 31985062
Fetal Bovine Serum (FBS)	Peak Serum	Cat# PS-FB3
L-Glutamine-200 mM (100×)	Thermo Fisher Scientific	Cat# 250-30-081
β-mercaptoethanol	Thermo Fisher Scientific	Cat# 21985023
Gentamycin	Sigma-Aldrich	Cat# G1272
Penicillin-streptomycin	Thermo Fisher Scientific	Cat# 10378016
ACK Lysing Buffer	Gibco	Cat# A1049201
PBS (1×) pH 7.4	Thermo Fisher Scientific	Cat# 10010-049
Polybrene Infection / Transfection Reagent	Sigma-Aldrich	Cat# TR-1003-G
TransIT®-293 Transfection Reagent	Mirus	Cat# MIR2700
0.05% Trypsin-EDTA (1×), phenol red	Life Technologies	Cat# 25300054
Phorbol 12-myristate 13-acetate (PMA)	Sigma-Aldrich	Cat# P8139
Ionomycin	Sigma-Aldrich	Cat# I0634
GolgiSTOP	BD	Cat# 554724, RRID: AB_2869012
Ampicillin	Sigma-Aldrich	Cat# A0166
Luria Broth Base	Invitrogen	Cat# 12795-027
LB Agar	Invitrogen	Cat# 22700-025
0.5 M EDTA	Invitrogen	Cat# AM9260G
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, 405 nm (Dilution 1:500)	Thermo Fisher Scientific	Cat# L34957
Recombinant Human TGF-beta 1 Protein	R&D	Cat# 240-B
Recombinant Mouse IL-6 Protein	R&D	Cat# 406-ML
Recombinant Mouse IL-1β Protein	R&D	Cat# 401-ML
Critical commercial assays
PureLink® HiPure Plasmid Midiprep Kit	Life Technologies	Cat# K210005
Naive CD4+ T Cell Isolation Kit, mouse	Miltenyi Biotec	Cat# 130-104-453
eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set	Thermo Fisher Scientific	Cat# 00-5523-00
Cell lines and bacteria strain
Human: Plat-E Retroviral Packaging Cell Line	Cell Biolabs, Inc.	Cat# RV-101
DH5α E. coli	Biomiga	Cat# CC1101-01
Experimental models: Organisms/strains
C57BL/6 RORγtWT and RORγtS182A mice (gender-matched 8–12-week-old littermates)	Ma et al., (2022)	N/A
Recombinant DNA
MSCV-IRES-NGFR (NGFR)	Addgene	RRID: Addgene_27489
MSCV-IRES-Thy1.1 (Thy1.1)	Addgene	RRID: Addgene_17442
Antibodies
Rabbit IgG Fraction to Hamster IgG (Whole Molecule) Antibody (Dilution 1:20)	MP Bio	Cat# 0855398, RRID: AB_2334935
PE anti-human CD271 (NGFR) Antibody (Clone ME20.4) (Dilution 1:400)	BioLegend	Cat# 345106, RRID: AB_2152647
Pacific Blue™ anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody (clone OX-7) (Dilution 1:400)	BioLegend	Cat# 202521, RRID: AB_1595584
APC/Cyanine7 anti-mouse IL-17A Antibody (clone TC11-18H10.1) (Dilution 1:100)	BioLegend	Cat# 506939; RRID: AB_2565780
PE/Cyanine5 anti-mouse CD4 Antibody (clone GK1.5) (Dilution 1:400)	BioLegend	Cat# 100410; RRID: AB_312695
CD3ε Monoclonal Antibody (clone 145-2C11), Functional Grade (Dilution 1:1000)	Thermo Fisher Scientific	Cat# 16-0031-86, RRID: AB_468849
CD28 Monoclonal Antibody (clone 37.51), Functional Grade (Dilution 1:4000)	Thermo Fisher Scientific	Cat# 16-0281-86, RRID: AB_468923
ROR gamma (t) Monoclonal Antibody (clone B2D), PE-eFluor 610 (Dilution 1:200)	Thermo Fisher Scientific	Cat# 61-6981-82; RRID: AB_2574650
IL-10 Monoclonal Antibody (clone JES5-16E3), Alexa Fluor 488 (Dilution 1:100)	Thermo Fisher Scientific	Cat# 53-7101-80; RRID: AB_469925
IL-10 Monoclonal Antibody (clone JES5-16E3), Alexa Fluor 700 (Dilution 1:100)	Thermo Fisher Scientific	Cat# 56-7101-80; RRID: AB_891570
FOXP3 Monoclonal Antibody (clone FJK-16s), PE-Cyanine5.5 (Dilution 1:200)	Thermo Fisher Scientific	Cat# 35-5773-82; RRID: AB_11218094
ChromPure Rat IgG (Dilution 1:50)	Jackson ImmunoResearch	Cat# 012-000-013; RRID: AB_2337136
Software and algorithms
FlowJo™ v10.8	BD	https://www.flowjo.com/
Other
Biological Safety Cabinet	Thermo Fisher Scientific	1384-M
Centrifuge	Eppendorf	5810R
NanoDrop Lite	Thermo Fisher Scientific	BZB8917251
ZE5 Cell Analyzer	Bio-Rad	12004278
15 mL Conical tubes	Corning	Cat# 188261
50 mL Conical tubes	Corning	Cat# 227270
24-well tissue culture plate	Fisher Scientific	Cat# 353047
6-well tissue culture plate	Eppendorf	Cat# 30720113
10 mL serological pipettes	Genesee Scientific	Cat# 12-112
5 mL serological pipettes	Genesee Scientific	Cat# 12-110
3 mL syringe	Fisher Scientific	Cat# 14823436
Millex-HV Syringe Filter Unit, 0.45 μm	Fisher Scientific	Cat# SLHV033RS
40 μm cell strainer	Falcon	Cat# 352340
Curved forceps	Denville	Cat# S728685
Delicate tip straight forceps	Denville	Cat# S728696
Micro dissecting scissors 3.5″	Roboz Surgical Store	Cat# RS-5910
Vi-Cell cell counter	Beckman Coulter	AZ41595