TABLE 1.
Bacterial strain | Reduction in viable cell or spore no. (log10 CFU/ml) under the following conditionsa:
|
||||||
---|---|---|---|---|---|---|---|
5°C, 25 min | 5°C + PEFb, 25 min | 20°C, 25 min | 20°C + PEF, 25 min | 50°C, 25 min | 50°C + PEF, 25 min | 72°C, 25s | |
M. paratuberculosis ATCC 19698 | 0 a | 1.5 ± 0.2 f | 0 a | 3.1 ± 0.2 h | 0.01 ± 0.01 b | 5.1 ± 0.3 j | 2.4 ± 0.3 g |
M. paratuberculosis Linda (ATCC 43015) | 0 a | 1.7 ± 0.4 f | 0 a | 3.3 ± 0.3 h | 0.02 ± 0.005 b | 5.3 ± 0.5 j | 2.8 ± 0.2 g |
L. monocytogenes 11994 | 0 a | 1.1 ± 0.1 e | 0 a | 2.6 ± 0.1 g | 0.16 ± 0.05 d | 4.1 ± 0.3 i | 7.1 ± 0.2c k |
B. cereus 11145 (endospores) | 0 a | 0.16 ± 0.02 d | 0 a | 0.14 ± 0.03 d | 0 a | 0.17 ± 0.04 d | 0.06 ± 0.02 c |
Values followed by the same lowercase letter do not differ at the 95% confidence interval (P < 0.05). Results are means and standard deviations.
Treatment of suspended bacterial cells with 2,500 pulses at 30 k V/cm.
Reduction of starting population of 7.1 ± 0.2 log10 CFU/ml to a nondetectable level when assessed after 15 s at 72°C.