Table 1.

Summary of direct membrane mosquito-feeding assays grouped by clinical and diagnosis status

Characteristic	Symptomatic, microscopic (n = 57)	Asymptomatic, microscopic (n = 18)	Asymptomatic, sub-microscopic (n = 30)	Overall (N = 105)
Gender, n (%)
Female	25 (44)	8 (44)	12 (40)	45 (43)
Male	32 (56)	10 (56)	18 (60)	60 (57)
Parasite density, parasites/μL; median (IQR)	2,700 (772–5,320)*	131.5 (63–326.5)*	7.76 (3.23–14.43)†	1,570 (143–4,054)
Gametocyte density, gametocytes/μL; median (IQR)	448 (120–669)	24 (3–48)	–	352 (36– 618)
No. of infectious individuals	44	4	0	48
Total oocysts/infected mosquito, median (IQR)	211 (32.25–1,098.25)	5 (4.25–15.50)	0 (0–0)	167.5 (22.25–988)
Mosquito infection prevalence,‡ %; median (IQR)	76 (40–91.19)	7.22 (5.4–19.16)	0 (0–0)	74.75 (33.52–90.28)
Intensity of infection, mean ± SD	22.14 ± 29.96	0.65 ± 1.13	0	20.35 ± 29.28

^*Parasite density in symptomatic and asymptomatic microscopic groups was calculated with microscopy only.

^†Parasite density in the symptomatic sub-microscopic group was quantified using quantitative polymerase chain reaction methods, but we could not differentiate sexual stage from asexual stage.

^‡Mosquito infection prevalence calculated for positive infections only.