Table 2.
Results of paired direct membrane mosquito-feeding assays and skin-feeding assays of symptomatic Plasmodium vivax participants (N = 27)
| Characteristic | Skin-feeding assay | Direct membrane mosquito-feeding assay |
|---|---|---|
| Gender, n (%) | ||
| Female | 8 (29.6) | |
| Male | 19 (70.4) | |
| Parasite density, parasites/μL; median (IQR) | 3,000 (853–5,452) | |
| Gametocyte density, gametocytes/μL; median (IQR) | 498 (356–679.5) | |
| Proportion of infected feeds, % (n/N) | 88.8 (24/27) | 88.8 (24/27) |
| Fed mosquitoes, %; median (IQR) | 85 (70–97.2) | 83.3 (73.3–93.3) |
| Infected mosquitoes, % (n/N) | 48.5 (248/511) | 58.9 (308/523) |
| Total oocysts/infected mosquito, median (IQR) | 73 (4.75–276.75) | 140 (27.25–464.25) |
| Sporozoites/mosquito, median (IQR) | 2,405 (909–13,706) | 2,750 (400–20,000) |
| Mosquito infection rate, %; median (IQR) | 55.26 (15–81.25) | 85 (40–91.25) |
| Intensity of infection, mean ± SD | 3.32 ± 11.94 | 19.12 ± 26.84 |
IQR = interquartile range. Parasite and gametocyte densities were calculated by microscopy. Dissections were performed in paired direct membrane mosquito-feeding assays and skin-feeding assays (N = 27), and sporozoite calculations were performed with pools of mosquitoes (range, 9–35; N = 848 mosquitoes dissected). The median sporozoite number per mosquito per participant was calculated in 14 paired experiments. The percentage of infected mosquitoes was calculated only with positive infections.