Fig. 2. Test layouts for various SARS-CoV-2 detection methods.

a Demonstration of different applications using Epidax® including: SARS-CoV-2 confirmatory test using RT–qPCR, and screening using endpoint RT–PCR and RT-LAMP; b Layout for RT–qPCR and endpoint RT–PCR test using 4-channel chip with singleplex RT–PCR reagent and RNA extract sample; and interpretation of test results. One sample was tested for each chip using FAM as fluorescence probe. Channels 1 and 2 for NC and PC, respectively; channel 3 for N1 gene of SARS-CoV-2; and channel 4 for human RP gene (IC). Green color represents positive detection while gray color represents a negative one; c Layout for direct RT–qPCR and endpoint RT–PCR tests using 4-channel chip with multiplex direct RT–PCR reagent and nasopharyngeal sample in UTM. Two samples (in channels 2 and 3, respectively) were tested in each chip (demonstrating the ability of Epidax® to test multiple samples); channels 1 and 4 for NC and PC. For each channel, three target genes were detected using different fluorescence probes, particularly E gene of SARS-CoV-2 (FAM), N gene of SARS-CoV-2 (HEX) and RNase P (Texas Red). Gray represents a negative signal, and other colors (green, light green, and red) represent a positive signal; d Layout for RT-LAMP using 6-channel chip with RNA extract; and interpretation of results. Two samples were tested for each chip. Channels 1 and 2 for NC and PC, respectively. Channels 3, 4, 5, and 6 tested the N gene of SARS-CoV-2 and human rActin gene (IC) of two different samples, respectively. Yellow color indicates a positive detection, and pink color a negative reaction.