FIG. 2.

pGXIS construction. A and B are sequences in FMV DNA that were incorporated into the 3′ termini of primers CiH2 and CiH3; C and D are the sequences from primers 2 and 3 that specifically amplify a fragment in the X. axonopodis pv. citri plasmid (7) and are placed at the 5′ termini in primers CiH2 and CiH3. CiH2 (C plus A) and CiH3 (D plus B) were used to amplify the fragments from FMV DNA. The PCR product (400 bp) was cloned into the pGEM-T vector to result in pGXIS. In quantitative PCR, primers 2 (C) and 3 (D) were used for amplifications.