Table 1.

Primers for real-time quantitative PCR.

Gene	Primer sequence, 5′-3′	Source	Size (bp)
GAPDH	F: GGGTCATCATCTCTGCACCT R: GGTCATAAGTCCCTCCACGA	NM_001034034.2	176
IL-1β	F: CAGTGCCTACGCACATGTCT R: AGAGGAGGTGGAGAGCCTTC	NM_174093.1	209
IL-6	F: CACCCCAGGCAGACTACTTC R: TCCTTGCTGCTTTCACACTC	NM_173923.2	129
TNF-α	F: GCCCTCTGGTTCAGACACTC R: AGATGAGGTAAAGCCCGTCA	NM_173966.3	192
CXCL5	F: TGAGACTGCTATCCAGCCG R: AGATCACTGACCGTTTTGGG	NM_174300.2	193
CCL5	F: CTGCCTTCGCTGTCCTCCTGATG R: TTCTCTGGGTTGGCGCACACCTG	NM_175827	217
CCL2	F: GCTCGCTCAGCCAGATGCAA R: GGACACTTGCTGCTGGTGACTC	NM_174006	171
CXCL2	F: CCCGTGGTCAACGAACTGCGCTGC R: CTAGTTTAGCATCTTATCGATGATT	NM_174299.3	204
CXCL8	F: TGGGCCACACTGTGAAAAT R: TCATGGATCTTGCTTCTCAGC	NM_173925.2	136
ZO-1	F: TCTGCAGCAATAAAGCAGCATTTC R: TTAGGGCACAGCATCGTATCACA	XM_010817146.1	187
Occludin	F: GAACGAGAAGCGACTGTATC R: CACTGCTGCTGTAATGAGG	NM_001082433.2	122
Claudin 1	F: CGTGCCTTGATGGTGAT R: CTGTGCCTCGTCGTCTT	NM_001001854.2	102
Claudin 4	F: CTTCATCGGCAGCAACATC R: ACAACAGCACGCCAAACA	NM_001014391.2	191
TLR2	F: CAGGCTTCTTCTCTGTCTTGT R: CTGTTGCCGACATAGGTGATA	NM_174197.2	140
TLR4	F:GACCCTTGCGTACAGGTTGT R:GGTCCAGCATCTTGGTTGAT	NM_174198.6	103
CD14	F: CAGTATGCTGACACAATCAA R: AGTTCCTTGAGACGAGAGTA	NM_174008.1	122
MD2	F: GGAGAATCGTTGGGTCTGC R: GCTCAGAACGTATTGAAACAGGA	NM_001046517.1	92
MyD88	F: TCATTGAGAAGAGGTGCCGT R: TGGCTTGTACTTGATGGGGAT	NM_001014382.2	146
IRAK1	F: CCTCAGCGACTGGACATCCT R: GGACGTTGGAACTCTTGACATCT	NM_001040555.1	103
IRF3	F: TTGTGAACTCAGGAGTCAGG R: TGGGCTCAAGTCCATGTCAC	NM_001029845.3	125

F, forward; R, reverse.