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. 2022 Jul 4;18(11):4452–4465. doi: 10.7150/ijbs.69882

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Acetylation at K311 inhibits GAC activity. A-C NSCLC cells (H1299, A549, H292) were transfected with V5-tagged GAC and treated with NAM and/or TSA. Western blot assay was performed. WCL: whole cell lysate. D-F NSCLC cells (H1299, A549, H292) were treated with NAM and/or TSA. The protein expression was determined by western blot and glutaminase activity assay was performed. G V5-GAC^WT or V5-GAC^mutants (K311Q, K320Q, K328Q, K396Q) plasmids were transfected into H1299 cells and glutaminase activity assay was performed. H, I H1299 (H) and A549 (I) cells were treated with NAM and TSA. GAC K311-acetylation was detected by western blot assay using anti-GAC AcK311 antibody. Data are showed as mean ± SD, n=3. *P < 0.05, **P < 0.01.