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. 2022 Jul 4;18(11):4452–4465. doi: 10.7150/ijbs.69882

Figure 5.

Figure 5

Lys311 acetylation promotes GAC-TRIM21 interaction and GAC ubiquitination. A Indicated plasmids were transfected into H1299 cells. Interaction between GAC and TRIM21 were detected by immunoprecipitation and western blot assay. B Indicated plasmids were transfected into H1299 cells followed by treatment with or without NAM and TSA. Interaction between GAC and TRIM21 were detected by immunoprecipitation and western blot assay. C Indicated plasmids were transfected into H1299 cells. Interaction between GAC and TRIM21 were detected by immunoprecipitation and western blot assay. D Indicated plasmids were transfected into H1299 cells. Interaction between GAC and TRIM21 were detected by immunoprecipitation and western blot assay. E Indicated plasmids were transfected into H1299 cells. The levels of GAC ubiquitination were detected by immunoprecipitation and western blot assay. WCL: whole cell lysate.