Figure 6.

GAC acetylation inhibits NSCLC progression in vivo. A A549-GAC^WT and A549-GAC^K311Q stable cells were cultured for indicated times, then cell proliferation assay was performed. The expression of indicated proteins was determined by western blot with the indicated antibodies. B A549-GAC^WT and A549-GAC^K311Q stable cells were seeded in 6-well plates and colony formation assay was performed. C A549-GAC^WT and A549-GAC^K311Q stable cells were cultured in RPMI 1640 and soft agar assay was performed. Colonies were photographed after 14 days of growth. Scale bars of top figures is 20 µm. Scale bars of bottom figures is 50 µm. D, E Xenograft tumorigenesis. Nude mice were subcutaneously injected with parental A549-GAC^WT and A549-GAC^K311Q stable cells (1×10⁷). Tumors were dissected out and photographed (D) after four weeks, and then tumor weights and volumes were measured (E). F Tumors formed by parental A549-GAC^WT or A549-GAC^K311Q stable cells were subjected to hematoxylin-eosin (H&E) staining. Scale bars of top figures is 50 µm. Scale bars of bottom figures is 10 µm. G, H Immunohistochemical staining of tumors formed by A549-GAC^WT or A549-GAC^K311Q stable cells for Ki67 and TTF1. Scale bars of figures are 50 µm. Data are showed as mean ± SD, n=3. *P < 0.05, **P < 0.01, ***P < 0.001.