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. 2022 Jun 16;11(7):2518–2522. doi: 10.1021/acssynbio.2c00093

Figure 1.

Figure 1

TcI mutants act as tunable, temperature-sensitive transactivators. (a,b,c) Circuit diagrams of gene activation constructs. TcIx (x = 38, 39) (a), wildtype CI (b), or no activator (c) activates expression of mWasabi (GFP) from the PRM promoter. (d,e,f,g) Summed frequency histograms for GFP channel for expression of GFP from PRM promoter by TcI38 (d), TcI39 (e), wildtype cI (f), or at baseline (no activator) (g). NF indicates nonfluorescent control measured in the same channel. (h) Thermal profile of mean population fluorescence of GFP expressed from the PRM promoter with activation by TcI38, TcI39, and wildtype CI, or at baseline (no activator) in E. coli. (i) Thermal profile of % wildtype activation of gene expression by TcI38 and TcI39. At each temperature, 100% wildtype activation indicates expression equal to wildtype CI, and 0% activation indicates expression equal to unactivated PRM. Eight hours of incubation, n = 4 biological replicates. Error bars represent ± SEM.