Figure 3.
CRISPR/LbCas12a genome-edited replacement of the M. maripaludis flagellum operon with the E. coli β-glucuronidase gene (uidA). Shown are the transformation efficiencies [cfu (2 μg DNA)−1] for the CRISPR/LbCas12a pMM002P-derived plasmids that were used to transform M. maripaludis. Plasmids p002-218 and p002-226 (controls) express one and two gRNAs, respectively, and do not contain the RF. Plasmids p002-218-uidA and p002-226-uidA express one and two gRNAs, respectively, but contain the RF. Error bars represent the standard deviation of the values obtained for the transformation efficiency (n = 3). Positive rates representing the fraction of correctly edited colonies per colonies tested by PCR are shown for the p002-218-uidA and p002-226-uidA transformations (numbers above bars).