Fig. 1.

In vivo experimental setup. (a) Murine histotripsy experiments were conducted using a 1-MHz transducer. (b) Timing for treatment, euthanasia, and data collection were set as diagramed. Days with histology collection are noted by cassettes, flash-frozen tumors noted by tubes with tumors, and serum noted by tubes with serum, and flow cytometry is noted by flow charts. Tumors located with ultrasound imaging were used for (c) planning automated ablation disks and (d) raster scan plots. (e) Therapy was guided by coaxially aligned ultrasound imaging. The red arrow indicates a bubble cloud that was generated during treatment.