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. 2022 Jul 19;24:170. doi: 10.1186/s13075-022-02860-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — RNA-seq analysis for pSS neutrophils (pSS=7, HC=6). A The volcano plot for DE mRNAs of pSS neutrophils (239 upregulated in red and 181 downregulated mRNAs in blue, TOP 5 up- and downregulated genes were annotated). B The hierarchical clustering heatmap for DE mRNAs of the pSS neutrophils. C GO enrichment analysis for DE mRNAs of pSS neutrophils (implemented by the clusterProfiler R package). D GSEA for the potential function of DE mRNAs of pSS neutrophils. E KEGG analysis for the potential pathways of pSS neutrophils’ DE mRNAs. Different colors represented for the different signaling pathways. *P-value < 0.05, **P-value < 0.01, ***P-value <0.001)