Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 19;24:170. doi: 10.1186/s13075-022-02860-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Results for rhIFN-α stimulation of neutrophils in pSS patients and healthy controls. A Results MitoSOX measurements of pSS and HC plasma stimulation of healthy neutrophils (n=9). B Representative flow cytometry results for the JC-1 staining in pSS patients and healthy controls with or without rhIFN stimulation (HC\pSS-Unstimulated represented for the freshly isolated neutrophils, pSS\HC-with or without rhIFN represented for the neutrophils stimulated with or without rhIFN). C Representative flow cytometry results of ROS production in pSS and healthy neutrophils with or without rhIFN stimulation (HC\pSS-Unstimulated represented for the freshly isolated neutrophils, pSS\HC-with or without rhIFN represented for the neutrophils stimulated with or without rhIFN, different color represented for the different subjects). D Comparison of JC-1 monomer percentage (mitochondrial damage, n=10), ROS production (n=10), and MPO concentration (n=12) between neutrophils stimulated with or without rhIFN-α. E Immunofluorescence staining of NETs-DNA (DAPI, blue) with or without rhIFN stimulation. F IFN-α receptor inhibitor reduced the mitochondrial damage (MitoSOX) and the production of NETs (SytocGreen and MPO levles). *P-value < 0.05, **P-value < 0.01, ***P-value <0.001)