Table 5:

Summary of key guidelines and recommendations proposed within the 4.1 Arthropod community sample DNA amplification, library preparation, and sequencing submodule

4.1 Arthropod community sample DNA amplification, library preparation, and sequencing submodule
Target DNA fragments and primers	COI locusSecond half (3′) of the COI barcode fragmentDegenerate primers (see Elbrech et al. 2019)
PCR conditions	Minimize number of PCR cyclesDilution of DNA extractNonproofreading TaqPCR replicates (3), ideally individually labeledNegative controlsTechnical PCR replicationCross-contamination control practices
Library preparation	Two-step protocol