Table 2:

NovoSpaRc: modes offered by spacemake and their outcome based on data availability

Data produced	Publicly available data	NovoSpaRc mode	Outcome
–	scRNA-seq	De novo	Basic spatial reconstruction
–	scRNA-seq + spatial	With markers	Enhanced gene counts
scRNA-seq	–	De novo	Basic spatial reconstruction
scRNA-seq	Spatial	With markers	Enhanced gene counts
Spatial	scRNA-seq	With markers	Enhanced gene counts
Spatial + scRNA-seq	–	With markers	Enhanced gene counts