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. Author manuscript; available in PMC: 2022 Jul 19.
Published in final edited form as: Biochemistry. 2019 Jun 18;58(26):2906–2920. doi: 10.1021/acs.biochem.8b01316

Figure 2.

Figure 2.

SAXS model of 5XAla-NEMO. (A) Scattering curve for 5XAla-NEMO (blue), with theoretical scattering (red) calculated from the model shown in panel D. Inset: the error-weighted residual difference plot, Δ/σ = [I exp(q) − cI mod(q)]/σ(q) versus q for model fitting to experimental scattering. (B) Guinier plot. Linearity at low q is not necessarily expected for a partially flexible, extended protein50,51. (C) Distance-distribution function generated by GNOM. (D) Shape reconstruction generated by DAMMIN (gray envelope) with superposed NEMO model (blue) generated from three NEMO domains by BUNCH. “N-term” and “C-term” labels denote NEMO N terminus and C terminus, respectively; the Intervening Domain is denoted “IVD.” (E) Guinier plots showing lack of aggregation with increasing protein concentration for WT-NEMO.