Table 2.
Comparison of clinical and laboratory indicators between the two different DNA fragmentation index groups
| Indicator | Control group | MACS group | Z/χ2 | P |
|---|---|---|---|---|
| Total number of oocyte-retrieved cycles (n) | 41 | 39 | ||
| Dosage of Gn used (IU), mean±s.d. | 1914.0±557.4 | 1986.2±642.2 | 0.54 | 0.59a |
| Duration of Gn used (day), mean±s.d. | 10.8±2.0 | 11.5±2.3 | 1.55 | 0.12a |
| Number of oocytes retrieved (n), mean±s.d. | 13.0±5.0 | 13.7±6.4 | 0.52 | 0.60a |
| Normal fertilization rate, % (n/total) | 77.1 (361/468) | 76.6 (321/419) | 0.03 | 0.85 |
| Cleavage rate, % (n/total) | 98.3 (355/361) | 97.8 (314/321) | 0.24 | 0.62 |
| Blastocyst formation rate, % (n/total) | 65.4 (151/231) | 66.5 (131/197) | 0.06 | 0.80 |
| Transplantable embryo rate, % (n/total) | 59.7 (212/355) | 61.8 (194/314) | 0.29 | 0.59 |
| No available embryo rate, % (n/total) | 4.9 (2/41) | 2.6 (1/39) | 0.29 | 0.58 |
| Number of good quality embryos transferred of first embryo transfer cycles (n), mean±s.d. | 1.3±0.6 | 1.5±0.5 | 1.16 | 0.25a |
| Clinical pregnancy rate of first embryo transfer cycles, % (n/total) | 59.0 (23/39) | 65.8 (25/38) | 0.38 | 0.54 |
| Implantation rate of first embryo transfer cycles, % (n/total) | 55.6 (30/54) | 53.6 (30/56) | 0.04 | 0.83 |
| Early miscarriage rate of first embryo transfer cycles, % (n/total) | 5.1 (2/39) | 2.6 (1/38) | 0.32 | 0.57 |
| Twin pregnancy rate of first embryo transfer cycles, % (n/total) | 30.4 (7/23) | 20.0 (5/25) | 0.70 | 0.40 |
| LBR of first embryo transfer cycles, % (n/total) | 53.9 (21/39) | 63.2 (24/38) | 0.68 | 0.41 |
| Mean transfer number of each retrieved cycle (n), mean±s.d. | 1.6±0.8 | 1.2±0.5 | 2.25 | 0.02*,a |
| Mean transfer embryo number of each retrieved cycle (n), mean±s.d. | 2.3±1.6 | 1.7±0.7 | 2.14 | 0.03*,a |
| Cumulative LBR, % (n/total) | 70.7 (29/41) | 79.5 (31/39) | 0.82 | 0.37 |
*P<0.05, aMann–Whitney U test, Z/χ2 value represented the statistical results of Mann–Whitney U test or Chi-square test. Cleavage rate (%)=number of cleavage-stage embryos on day 2/number of normal fertilized oocytes ×100%; blastocyst formation rate (%)=number of blastocyst formation on day 5 and day 6/number of cultured cleavage-stage embryos on day 3 ×100%; transplantable embryo rate (%)=available number of transplantable embryos/2PN cleavage-stage embryos ×100%; cumulative LBR (%)=number of live births in each egg retrieval cycle (fresh and frozen-thawed embryos transfer)/total number of oocyte retrieval cycles ×100% (the follow-up lasted for 18–36 months from the day of oocyte retrieval; and during the follow-up period, frozen embryos were not collected from patients without live births). LBR: live birth rate; 2PN: 2 pronucleus; MACS: magnetic activated cell sorting; s.d.: standard deviation