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. 2021 Nov 5;24(4):359–366. doi: 10.4103/aja202154

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Copyright: ©The Author(s)(2021)

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Confirmation of the differentially expressed proteins in SPEF2-mutant spermatozoa. The expression of the randomly selected proteins in the spermatozoa of the normal control (NC) and SPEF2-mutant individuals. The western blot results confirmed the increased expression of IFT20 and the decreased expression of SPEF2, SPAG6, RSPH4A, RSPH1, TOM20, SPACA1, EFHC1, DYNLT1 and MNS1 in the spermatozoa of the SPEF2-mutant individuals compared with those of the NC. ACTIN and GAPDH were used as loading controls. Data are representative of three independent assays. SPEF2: sperm flagellar protein 2; SPAG6: sperm-associated antigen 6; RSPH4A: radial spoke head protein 4; RSPH1: radial spoke head 1; TOM20: translocase of outer mitochondrial membrane 20; SPACA1: sperm acrosome associated 1; EFHC1: EF-hand domain containing 1; DYNLT1: dynein light chain tctex-type 1; MNS1: meiosis-specific nuclear structural protein 1; ACTIN: actin beta; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IMT: intramanchette transport; IFT: intra-flagellar transport.