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. 2021 Nov 5;24(4):359–366. doi: 10.4103/aja202154

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Copyright: ©The Author(s)(2021)

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The interaction between RSPH9 and SPEF2 was identified by co-IP assay in vitro. HEK293T cells were co-transfected with vectors encoding FLAG-SPEF2 and HA-RSPH9. HEK293T cells were transfected with the vector encoding HA-RSPH9 as a negative control. Cell lysates were immunoprecipitated (IP) with an anti-FLAG antibody. The resultant protein samples were separated by SDS-PAGE and analyzed by immunoblotting (IB) with antibodies against FLAG and HA. Data are representative of three independent assays. RSPH9: radial spoke head component 9; SPEF2: sperm flagellar protein 2; FLAG: DYKDDDDK peptide; HA: haemagglutinin; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis.