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. 2022 Jul 18;19:190. doi: 10.1186/s12974-022-02552-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4. — Ex vivo phagocytosis assays in MDMs and microglia in the post-ischemic brain. For phagocytosis assays, the cells were isolated in the post-ischemic brain of asplenic mice that received GFP + splenocytes and bead^580/605 at 6th day after stroke. a Gating strategy for isolated cells containing GFP and /or bead^580/605 with subsets P1–P4 containing bead⁺ phagocytes. b Measures of phagocytic activity in GFP⁻ (P1, P3) and GFP⁺ (P2, P4) cells within the contralateral (P1, P2) and ipsilateral (P3, P4) hemispheres. Phagocytic activity was monitored by counts of cells containing bead^580/605 (counts), mean fluorescence intensity (MFI) of bead⁺ cells, and a phagocytic index (counts x MFI). c Cell count distribution of P1’–P4’ subsets, which contain CD45⁺/CD11b⁺/NK1.1⁻/Ly6G⁻ microglia and MDMs. d Measures of phagocytic activity for microglia (GFP⁻) and MDMs (GFP⁺) in the contralateral (P1’, P2’) and ipsilateral (P3’ P4’) hemispheres. Measures of statistical significance were determined by one-way ANOVA with post hoc Bonferroni comparison test (^**, ^***, and ^****, respectively, indicating p < 0.01, 0.001, 0.0001)