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. 2022 Jun 21;204(7):e00182-22. doi: 10.1128/jb.00182-22

FIG 1.

FIG 1

Transposon mutagenesis of rarp-1 impairs R. parkeri infection. (A) R. parkeri RARP-1 contains an N-terminal Sec secretion signal (SS; orange), a central intrinsically disordered region (IDR; light blue), and C-terminal ankyrin repeats (ANKs; dark blue). Tn insertions at residues 305 (Sp116) and 480 (Sp64) are indicated (arrowheads). (B) Plaque areas in infected Vero cell monolayers. Means from two independent experiments (squares) are superimposed over the raw data (circles) and were used to calculate means ± standard deviations (SD) and P values (unpaired two-tailed t test). *, P < 0.05 relative to WT. (C) Western blot for RARP-1 using purified R. parkeri strains. 3×FLAG-tagged and endogenous RARP-1 are indicated (arrowheads). OmpA, loading control. (D) Western blot for FLAG using purified R. parkeri strains. 3×FLAG-tagged RARP-1 is indicated (arrowhead). OmpA, loading control.