FIG 3.

RARP-1 is dispensable for evasion of host cell autophagy and supports host cell invasion. (A) Recruitment of LC3 during infection of A549 cells. Samples were stained for LC3 (magenta) and bacteria (cyan). The ompB::Tn mutant was used as a positive control, and bacteria associated with LC3-positive membranes are indicated (arrowheads). Scale bar, 2 μm. (B) Efficiency of invasion into A549 cells. The means ± SD from a representative experiment (n = 20 fields of view each with ≥45 bacteria) were compared at each time point (one-way ANOVA with post hoc Dunnett’s test). **, P < 0.01; ****, P < 0.0001 relative to WT.