FIG 1.

M. maripaludis expressing FAST is fluorescent upon HMBR addition. (A) Fluorescence intensities of M. maripaludis strains cultivated in McCas medium with H₂ as the electron donor for growth. HMBR was added to a final concentration of 10 μM. Relative fluorescence units were determined by normalizing emission readings from a microplate reader against the baseline autofluorescence of the sample without fluorogen. Values were also normalized to the OD₆₀₀ of the culture. (B) Titration of HMBR in cells expressing FAST1 or the tandem variant tdFAST2 grown in McCas medium with H₂. Values were normalized to the OD₆₀₀ of the culture. Data are averages and standard deviations from triplicate measurements.