FIG 4.

BiFC and fluorescence of Fdh1 with splitFAST. (A) Fluorescence intensities of cells expressing various splitFAST constructs. The negative-control strains expressing fdhA-NFAST and mtd-CFAST (168 cells) and mtd-NFAST and fdhB-CFAST (160 cells) were compared to the strain expressing fdhA-NFAST and fdhB-CFAST (190 cells). Data were obtained using microscopy and are means and standard deviations from samples collected at least in triplicate. (B) Fluorescence intensities of cells expressing fdhA-NFAST and fdhB-CFAST grown in medium with either formate (190 cells) or H₂ (183 cells) as the sole electron donor. Different circle colors represent data points from separate replicates, and mean values from all averaged cells in a replicate are represented by large circles. Data were obtained using microscopy and are means and standard deviations from quadruplicate samples.