FIG 2.

ispA genomic landscape. (A) Circular genome representation of MP10 (SCV) created using CGView (32). The inner ring illustrates the GC skew. The outer two rings represent coding sequences (CDS), tRNAs, and rRNAs on the reverse and forward strands, respectively. A black box is included to highlight the ispA gene. (B) Genomic localization of ispA. Nucleotide and protein alignments of PAO1 (GenPept accession number NP_250121), MP02, and MP10, showing the 12-bp deletion in MP10 and the consequent deletion of amino acids from 267 to 270. Protein and nucleotide sequence alignments were generated using Clustal Omega (33). (C) Schematic pathway displaying the involvement of IspA (also knowns as FPPS) in ubiquinone biosynthesis. Dotted arrows indicate that multiple steps have been abbreviated. FPP, farnesyl pyrophosphate; FPPS, farnesyl pyrophosphate synthase; G3P, glycerol-3-phosphate; GPP, geranyl pyrophosphate; MEP/DOXP, 2-C-methyl-d-erythritol 4-phosphate/1-deoxy-d-xylulose 5-phosphate; PP, pyrophosphate; TCA, tricarboxylic acid.