TABLE 2.
Oligonucleotide primers used in this study
| Primera | Sequence (5′ to 3′)b | Purpose |
|---|---|---|
| W11 | CTCCCCTGATTTTGTTAGGGATTTTC | Verify icsA locus |
| W12 | GCCATCACAGGAAGCAGCCTC | |
| W13 | GGATATAGAAGAGCGGTTTG | Verify virK locus |
| W14 | ACTTTATAATTTCAAGGGTACGGGTCCG | |
| W15 | GCACTTTGTGTACCTGCGATC | Verify icsP locus |
| W16 | GCACTATTTTTAATGGTGCCAG | |
| W17 | CGAATCGCTGCAGGATATTATGATGCTGGAGTTTTGCGAAGC | Verify virF locus |
| W18 | CGAATCGAATTCCCATCTGGCAATAGCGATGGGC | |
| W19 | CGAATCGAATTCTGAATTGGGCAGTTTACATCAGTG | Verify virB locus |
| W20 | CGAATCGCTGCAGATTCTCTTTCTCTGATTGAAATGCTGG | |
| W696 | CGCAGAgtcgacCACAGTATTCGGAACTAATTATAAAAGATAAATTATCCC | Make pPvirB(−1946)-lacZ |
| W746 | CGCAGAgtcgacGCCAATGAGAAAACATCCCAACC | Make pPvirB(−1437)-lacZ |
| W747 | CGCAGAgtcgacCTCATCAGATACAACTAAAAGTAGCGC | Make pPvirB(−976)-lacZ |
| W557 | CGCAGActgcagAATATGATAAAAGAAAAAATATTATCAATAGTGGC | Make pPvirB(−402)-lacZ |
| W558 | CGCAGActgcagCGTACAGCAAACTATCTGAAGAA | Make pPvirB(−350)-lacZ |
| W559 | CGCAGActgcagGATTCTCTTTCTCTGATTGAAATGC | Make pPvirB(−250)-lacZ |
| W560 | CGCAGActgcagTCTACGTATAGATGAATCTACATTAGAAC | Make pPvirB(−200)-lacZ |
| W561 | CGCAGActgcagTTATTTCTGTAGTCAAAAATAGTACAAAATCA | Make pPvirB(−116)-lacZ |
| W493 | CGCAGActgcagATAGTAATTTTTAAGACTACCGTTGAC | Make pPvirB(−58)-lacZ |
| W476 | TCTGCGtctagaATCACACCCTGTTTATTCATATTGAT | Reverse primer to make PvirB truncations |
| W690 | CGCAGAgtcgacCCGCATCCCCTAAACGGT | Make pPospF-lacZ |
| W691 | CGCTACtctagaCATTCAAAGAATCTAAATTTAGTTTTAGACAGGGCT | |
| W672 | CGCAGAgaattcCATGAACCAGTTAACGTTGAGC | Make pBAD18-mxiE and pBAD18-mxiE-ipgC |
| W677 | TCGATAATTCTCTTGCAGAAAAGCC | |
| W723 | CGCAGAgaattcACCATCCTTATAGAGAAGGATGGG | |
| W674 | CGCAGAgtcgacGCCGGATCCTTAATTAAGTCTAGAAC | Make pBAD18-mxiE |
| W675 | CGCAGAgtcgacCCCTAATAATTACTCCTTGATATCCTGAATTG | Make pBAD18-mxiE-ipgC |
a
Primers supplied by Integrated DNA Technologies.
b
Enzyme restriction sites PstI (in bold), XbaI (in italics), EcoRI (underlined), and SalI are in lowercase.