TABLE 3.
gBlock gene fragments used in this studya
| Name | Sequence (5′ to 3′) |
|---|---|
| gBlock 12b | ACGCCCcttaagGACTGGCGTATGGCAATGAGCTGCTTTATTATCAAGTTTGGTGATCGCCTTGACGGTCACTTCTGAGTGAAGGCGCTTGCACAGAATGGTAAACGGACTCGATTATTGAATCTTAGGGATAAGATATATCTACGATATGTCAATATACGAAATCGAAAGAATTTATTGGTGAATATAGCCATTCAATGTGGCAATGCAAAACCATGGTTAGCAATAGACTAATATCTATTACATTACAAAAATAGGTTATTGTTTTTTTTTAGCAAAAAGGATGAACAAAAATGTCAATAAATAACTATGGATTACATCCAGCAAACAACAAAAATATGCTCTAGATAATTT |
| gBlock 14c | ATATActgcagGCGAATTGGAGCTCCACCGCGGTGGCGGCCGCACTAGTGGATCCCCCGGGGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGATCTAGAATATA |
a
gBlock gene fragments are trademarked by Integrated DNA Technologies.
b
The mutated putative MxiE box at the ospD1 promoter is in gray shading. The restriction sites AflII (in lowercase) and XbaI (in bold) are indicated.
c
The multicloning site from pBluescript KS(+) II is in gray shading. The restriction sites PstI (in lowercase) and XbaI (in bold) are indicated.