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Representative flow cytometry analysis for CoVITEST. The flow cytometry gating strategy was performed sequentially; lymphocyte complexity (SSC vs. FSC), single cell, CD3+ and finally either CD4+ or CD8+ (A). From there, 40,000 CD4+ or CD8+ T cells were further evaluated for CD69 and IFN- γ expression in S and N peptide pool, negative control (vehicle) and positive control (SEB) samples to determine the T cell reactivity for each donor (B).
