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| Purification step | Total activity/ U | m(total protein)/mg | Specific activity/ (U/mg) |
Purification fold | Y/% |
|---|---|---|---|---|---|
| Crude supernatant | 209±14 | 120±12 | 1.7±0.1 | 1.0±0.0 | 100.0±0.0 |
| Ammonium sulfate precipitation | 76.6±2.3 | 14.4±1.2 | 5.3±0.2 | 3.06±0.09 | 36.7±1.1 |
| DEAE column chromatography | 41.9±0.4 | 2.8±0.1 | 15.0±0.2 | 8.60±0.09 | 20.1±0.2 |
DEAE=diethylaminoethyl cellulose
