Table S5. Results of gene expression are presented as the fold change in mRNA expression in a target sample, normalized to a reference gene and relative to the control sample.
| HCT-116 | E-cadherin | β-catenin | N-cadherin | vimentin | Snail |
|---|---|---|---|---|---|
| Control | 1 | 1 | 1 | 1 | 1 |
|
γ(royal jelly)/(µg/mL) 10 |
(3.2±0.2)ab | (1.2±0.3)a | (0.7±0.2)a | (0.75±0.09)a | (0.8±0.3)a |
| 100 | 1.3±0.2 | (1.4±0.2)ab | (0.20±0.02)ab | (0.48±0.04)ab | (0.72±0.08)ab |
|
c(10H2DA)/µM 10 |
(1.3±0.1)ab | (2.71±0.08)ab | (0.57±0.08)a | (0.4±0.1)a | (0.81±0.02)a |
| 100 | 1.0±0.4 | (2.37±0.02)a | (0.42±0.07)ab | (0.16±0.04)ab | (0.4±0.1)ab |
| SW-480 | E-cadherin | β-catenin | N-cadherin | vimentin | Snail |
| Control | 1 | 1 | 1 | 1 | 1 |
|
γ(royal jelly)/(µg/mL) 10 |
(1.35±0.08)a | (0.18±0.03)a | (0.13±0.03)ab | (0.69±0.09)a | (0.48±0.08)a |
| 100 | (2.89±0.04)ab | (0.61±0.03)ab | (0.15±0.03)a | (0.63±0.09)ab | (0.18±0.08)ab |
|
c(10H2DA)/µM 10 |
(1.41±0.03)a | (0.53±0.001)a | (0.11±0.001)a | (0.10±0.01)a | (0.35±0.02)a |
| 100 | (2.1±0.1)ab | (0.58±0.004)ab | (0.10±0.003)ab | (0.10±0.01)ab | (0.11±0.02)ab |
Values of relative gene expression were calculated according to 2-∆∆Ct method. ap<0.05 indicates a significant difference compared to control, while bp<0.05 indicates a significant difference between concentrations in treated group