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. 2022 Jul 15;10(7):e005063. doi: 10.1136/jitc-2022-005063

Figure 1.

Figure 1

Tumor cell-derived CCL2 is correlated with the polarization of TAMs, the trastuzumab resistance and the poor prognosis in HER2-positive GC patients. (A) The volcano plot was generated from mRNA sequencing analysis of HER2-negative and HER2-positive GC tissues. (B) Gene pathway analysis was performed and the candidate chemokine genes were shown. (C) IHC staining for CCL2 in GC tissues were shown. (D) The different IHC grades of CCL2 expression in HER2-negative and HER2-positive GC tissues was presented. (E) 33 HER2-positive patients were stratified into negative (−) and positive (+/++/+++) groups according to IHC staining for CCL2. Kaplan-Meier analysis of os in HER2-positive GC patients was performed based on CCL2 levels. (F) H&E staining and IHC staining for CCL2, CD68, CD86 and CD206 were shown to present the infiltrating TAMs in human HER2-positive GC tissues. (G–I) Pearson correlation analysis of IHC scores of CCL2 and CD68 (G), CCL2 and CD86 (H), as well as CCL2 and CD206 (I). (J) The immunofluorescence double staining for CD68 and CD206 was shown in human HER2-positive GC tissues. (K–M) 33 HER2-positive patients were categorized into negative (−) and positive (+) groups, based on the median point of IHC score of CD68, CD86 or CD206. Survival analysis of CD68 (K), CD86 (L) and CD206 (M) was showed. (N) The distribution of the trastuzumab resistance in HER2-positive GC patients. (O–R) The different IHC scores of CD68 (O), CD86 (P), CD206 (Q), and CCL2 (R) between the trastuzumab-sensitive and trastuzumab-resistance tissues were presented. scale bar, ×40, 500 µm; ×200, 100 µm. *P<0.05, ***p<0.001. GC, gastric cancer; IHC, immunohistochemistry; OS, overall survival.