Table 1.
LM profiles in peritoneal exudates of wild-type, Alox15-deficient, and α7nAChR-deficient mice subjected to VNS or sham treatment prior to zymosan-induced peritonitis
| Mediator | C57BL/6 | Alox15-deficient | α7nAChR-deficient | |||
|---|---|---|---|---|---|---|
| Sham | VNS | Sham | VNS | Sham | VNS | |
| RvD1 | 0.1 ± 0.3 | 0.4 ± 0.6 | 1.3 ± 0.3 | 0.5 ± 0.3 | 0.7 ± 0.3 | 6.3 ± 4.3 |
| RvD2 | 1.1 ± 0.8 | 5.5 ± 3.2** | 73.1 ± 40.7 | 0.9 ± 0.7 | 2.0 ± 0.6 | 13.1 ± 7.8 |
| RvD3 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.7 ± 0.4 |
| RvD4 | 2.6 ± 2.3 | 2.2 ± 1.4 | 2.4 ± 0.7 | 0.8 ± 0.5 | 1.1 ± 0.3 | 14.7 ± 9.4 |
| RvD5 | 2.0 ± 1.2 | 4.4 ± 2.5 | 1.2 ± 0.4 | 0.7 ± 0.3 | 2.3 ± 0.7 | 8.8 ± 2.6* |
| RvD6 | 0.7 ± 0.8 | 0.8 ± 1.4 | 2.5 ± 0.4 | 0.9 ± 0.6 | 2.3 ± 1.3 | 14.8 ± 6.2 |
| 17R-RvD1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.1 | 0.0 ± 0.0 | 0.1 ± 0.1 | 0.4 ± 0.2 |
| 17R-RvD3 | 0.3 ± 0.2 | 1.0 ± 0.9 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.2 |
| PD1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.7 ± 0.3 | 0.4 ± 0.2 | 0.5 ± 0.2 | 7.8 ± 5.3 |
| 17R-PD1 | 0.8 ± 0.4 | 1.8 ± 0.9* | 0.5 ± 0.3 | 0.2 ± 0.2 | 0.3 ± 0.2 | 0.8 ± 0.3 |
| 10S.17S-diHDHA | 26.3 ± 14.6 | 85.4 ± 48.8* | 2.3 ± 0.5 | 0.6 ± 0.5 | 8.5 ± 1.9 | 39.0 ± 13.9 |
| 22-OH-PD1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| MaR1 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.3 ± 0.2 | 0.1 ± 0.1 | 3.0 ± 0.8 | 9.2 ± 2.6 |
| MaR2 | 3.1 ± 2.5 | 17.0 ± 10.5* | 0.7 ± 0.4 | 0.0 ± 0.0 | 3.3 ± 0.8 | 14.2 ± 3.6* |
| 7S.14S-diHDHA | 3.2 ± 1.8 | 13.3 ± 10.3* | 1.9 ± 0.5 | 0.2 ± 0.1* | 3.8 ± 1.3 | 24.7 ± 9.0* |
| 4.14-diHDHA | 2.9 ± 1.3 | 9.0 ± 6.1* | 8.8 ± 1.3 | 3.1 ± 2.4 | 10.1 ± 5.0 | 51.5 ± 21.1 |
| RvT1 | 0.3 ± 0.3 | 0.9 ± 0.5 | 1.1 ± 0.2 | 0.4 ± 0.2 | 1.1 ± 0.4 | 4.8 ± 2.5 |
| RvT2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.1 | 0.0 ± 0.0 | 0.2 ± 0.2 |
| RvT3 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| RvT4 | 0.5 ± 0.4 | 0.5 ± 0.5 | 1.0 ± 0.4 | 0.8 ± 0.8 | 1.0 ± 0.5 | 12.3 ± 6.9 |
| RvD1n-3 DPA | 0.1 ± 0.1 | 0.1 ± 0.2 | 1.2 ± 0.2 | 0.2 ± 0.2* | 0.9 ± 0.4 | 5.1 ± 1.8 |
| RvD2n-3 DPA | 0.0 ± 0.0 | 0.0 ± 0.0 | 1.5 ± 1.5 | 0.0 ± 0.0 | 0.2 ± 0.1 | 0.3 ± 0.1 |
| RvD5n-3 DPA | 0.1 ± 0.3 | 0.6 ± 0.6 | 1.3 ± 0.3 | 0.2 ± 0.2* | 0.7 ± 0.4 | 8.1 ± 4.0 |
| PD1n-3 DPA | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| PD2n-3 DPA | 1.7 ± 1.0 | 4.6 ± 3.2 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.4 ± 0.2 | 2.5 ± 1.2 |
| 10S. 17S-diHDPA | 0.1 ± 0.1 | 1.1 ± 0.7** | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.9 ± 0.5* |
| MaR1n-3 DPA | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| MaR2n-3 DPA | 3.9 ± 0.8 | 4.0 ± 1.3 | 9.3 ± 0.7 | 10.5 ± 1.1 | 7.5 ± 0.7 | 11.7 ± 2.2 |
| 7S.14S-diHDPA | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.7 ± 0.1 | 0.3 ± 0.2 | 1.6 ± 0.2 | 8.5 ± 2.5 |
| RvE1 | 0.0 ± 0.1 | 0.2 ± 0.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.7 ± 0.2 |
| RvE2 | 1.4 ± 3.1 | 0.2 ± 0.5 | 0.3 ± 0.2 | 0.2 ± 0.2 | 0.8 ± 0.5 | 5.9 ± 2.5 |
| RvE3 | 0.0 ± 0.0 | 0.0 ± 0.0 | 1.3 ± 0.2 | 0.6 ± 0.2 | 1.9 ± 1.1 | 8.4 ± 4.6 |
| LXA4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 19.5 ± 16.8 | 7.8 ± 7.4 | 8.2 ± 3.7 | 128.9 ± 76.2 |
| LXB4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 167.1 ± 39.7 | 32.7 ± 23.2* | 51.7 ± 19.2 | 253.8 ± 134.3 |
| 5S.15S-diHETE | 16.0 ± 10.9 | 14.3 ± 11.9 | 29.6 ± 5.0 | 5.8 ± 2.9** | 28.0 ± 15.3 | 163.5 ± 62.2 |
| 15-epi-LXA4 | 47.4 ± 33.6 | 62.6 ± 88.6 | 130.4 ± 30.7 | 29.8 ± 15.3* | 78.9 ± 19.4 | 681.2 ± 424.1 |
| 15-epi-LXB4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 1.8 ± 1.6 | 3.7 ± 2.4 |
| LTB4 | 21.8 ± 4.0 | 68.9 ± 60.5 | 7.3 ± 0.8 | 4.6 ± 2.1 | 9.3 ± 3.6 | 51.5 ± 33.5 |
| 5S.12S-diHETE | 37.2 ± 14.3 | 89.6 ± 75.8 | 25.7 ± 6.3 | 6.6 ± 4.3* | 24.7 ± 9.7 | 161.1 ± 52.3* |
| 6-trans-LTB4 | 36.3 ± 10.0 | 89.1 ± 60.9 | 7.9 ± 0.7 | 2.9 ± 1.0** | 15.3 ± 4.2 | 60.6 ± 8.3** |
| 6-trans-12-epi LTB4 | 26.0 ± 11.1 | 75.2 ± 50.4* | 8.4 ± 1.2 | 2.9 ± 1.2* | 14.4 ± 2.9 | 54.9 ± 9.6** |
| 20-OH-LTB4 | 0.3 ± 0.2 | 0.3 ± 0.3 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.5 ± 0.2* |
| PGD2 | 155.6 ± 138.7 | 146.5 ± 66.7 | 9.2 ± 1.3 | 9.8 ± 1.6 | 4.2 ± 0.6 | 19.8 ± 10.4 |
| PGE2 | 354.6 ± 166.2 | 305.3 ± 90.8 | 41.3 ± 13.2 | 19.7 ± 4.2 | 23.3 ± 4.2 | 179.9 ± 122.4 |
| PGF2α | 67.4 ± 28.2 | 51.9 ± 14.8 | 17.3 ± 8.5 | 11.4 ± 1.9 | 8.4 ± 1.3 | 24.0 ± 6.7* |
| TxB2 | 121.2 ± 83.4 | 141.2 ± 38.5 | 11.0 ± 1.9 | 7.6 ± 0.7 | 5.6 ± 1.0 | 24.4 ± 9.7* |
Wild-type and Alox15- and α7nAChR-deficient mice were subjected to VNS or sham surgery and injected with zymosan (0.1 mg/mouse) intraperitoneally. Peritoneal exudates were collected 12 h after zymosan challenge. LM levels were measured using LC-MS/MS. Results are shown as mean ± SEM of picograms per exudate. *P < 0.05; **P < 0.01. Rv - Resolvin, MaR - Maresin, PD - Protectin, LX - Lipoxin, LT - Leukotriene, PG - Prostaglandin, Tx - Thromboxane, HETE - Hydroxyeicosatetraenoic acid.