Table 2.

Metabolic stability of CDD-956 and CDD-787 in MLM and HLM

	Assay (half-life), min*	CDD-787	CDD-956	JQ1	Alprazolam
1	MLM t1/2	13	35	13	302
2	HLM t1/2	33	139	10.3	864

Final concentrations: liver microsomes: 0.5 mg protein/mL, compound concentration: 2.0 μM, NADPH concentration: 1.0 mM; JQ1: short half-life control, Alprazolam: long half-life control. In duplicate at 0, 5, 10, 20, 40, and 60 min. HLM/MLM, human and mouse liver microsomes.

*The half-life less than 30 min in MLM and HLM was generally considered as unstable.