Figure 3.

PMP inhibition of KARs containing Neto1 or Neto2 auxiliary subunits. A, Representative currents from recombinant homomeric and heteromeric KARs containing either Neto1 or Neto2 auxiliary proteins (GluK1, top; GluK2, bottom). Currents were evoked by glutamate either alone (black lines) or in the presence of PMP (10 µm, colored lines) for either 1 s (GluK1/Neto2) or 100 ms (others). Top, The gray bar denotes glutamate application. GluK2/Neto1 and GluK2/Neto2 KARs were inhibited to a greater extent than GluK1 receptors with either Neto1 or Neto2. B, Normalized mean amplitudes of currents evoked from GluK1 and GluK1/GluK5 KARs with and without Neto proteins at a range of PMP concentrations were best fit with logistic curves with variable IC₅₀ and Hill slopes. The data and fitted curves for GluK1 and GluK1/GluK5 KARs are identical to those in Figure 2 and are shown for the sake of comparison. IC₅₀ values, 95% confidence intervals, and statistical analyses are shown in Table 1. C, Normalized mean amplitudes of currents evoked from GluK2 and GluK2/GluK5 KARs with and without Neto proteins at a range of PMP concentrations were best fit with logistic curves with variable IC₅₀ and Hill slopes. The data and fitted curves for GluK2 and GluK2/GluK5 KARs are identical to those in Figure 2 and are shown for the sake of comparison. IC₅₀ values, 95% confidence intervals, and statistical analyses are shown in Table 1.