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. 2022 Jul 5;13:921212. doi: 10.3389/fimmu.2022.921212

Figure 4.

Figure 4

CD160 engagement on hepatic NK cells enhances killing of allogenic CD8+ T cells. Activated CD3+ T cells co-cultured with H-NK cells with or without a mAb against CD160 for 24 hr (n = 13). (A), Schematic of experimental design. (B), Representative flow plots of the percent dead total T cells in each treatment group. ‘Ctrl’ indicates cocultures of CD3+ T cells with H-NK cells without the addition of any antibody. ‘aIgG1’ indicates cocultures of CD3+ T cells with H-NK cells with the addition of an IgG1 antibody. ‘aCD160’ indicates cocultures of CD3+ T cells with H-NK cells with the addition of an anti-CD160 mAb. (C–E), Percent dead total T cells (C), CD8+ T cells (D) and CD4+ T cells (E) following 24 hr co-culture of CD3+ T cells with hepatic NK cells. (F), Representative flow plots of HLA-A/B/C and HVEM MFI of CD8+ T cells at 0 hr and for each treatment at 24 hr. (G, H), MFI values for HLA-A/B/C (G) and HVEM (H) on CD8+ T cells and CD4+ T cells at 0 hr and for each treatment group at 24 hr. Data analysed using a Freidman test with Dunn’s multiple comparison (C–E) or a repeated measures one-way ANOVA (G–J). *p < 0.05, ** p < 0.01, and ***p < 0.001.