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. 2022 Jul 13;42(28):5482–5498. doi: 10.1523/JNEUROSCI.0086-22.2022

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Copyright © 2022 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Electrophysiological properties and subcellular distribution profiles of V5-labeled Na_v1.2 and Na_v1.6. A, WT (black) and V5-labeled (red) Na_v1.2 in HEK293T cells. Left, Na_v1.2 current examples. Right, Peak current density (top), channel activation (WT, n = 10; V5 labeled, n = 9), and steady-state inactivation (WT, n = 11; V5 labeled, n = 9) curves (bottom). B, WT (black) and V5 labeled (red) Na_v1.6 in HEK293T cells. Left, Na_v1.6 current examples. Right, Peak current density (top), channel activation (WT, n = 11; V5 labeled, n = 12) and steady-state inactivation (WT, n = 13; V5 labeled, n = 11) curves (bottom). C, Antiphase periodic striations of V5-labeled Na_v1.2 and actin in the AIS region of cultured hippocampal neurons. Right, top, Zoomed-in view of the rectangle region with dashed lines in the left image. Bottom, The intensity curves of Na_v1.2 (green) and actin (red) along the horizontal line. Scale bar, 1 µm. D, Analysis of Na_v1.2 (n = 12) and Na_v1.6 (n = 14) relative intensities along the dendrite and axon of cultured hippocampal neurons. Top, Curve shows the Na_v1.2/Na_v1.6 intensity ratio calculated by using Na_v1.2 and Na_v1.6 intensity data (middle). Error bars (shadow areas) represent SD.