Figure 4.

Dual labeling of Na_v1.2 and Na_v1.6 in the same neuron. A, The double-labeling strategy of Na_v1.2 and Na_v1.6 in the same neuron. Through sequential plasmid DNA electroporation and AAV virus infection 8 d later, double labeling of Na_v1.2 and Na_v1.6 in the same neuron was achieved. B, Labeling of Na_v1.2 with V5 tag and Na_v1.6 with HA tag in the same neuron (presentation, normal shading, Imaris). Scale bar, 10 µm. C, Super-resolution STED imaging of labeled Na_v1.2 and Na_v1.6 in the AIS region. Scale bar, 1 µm. Movie 1 shows the 3D image of a representative double-knock-in neuron.